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Differential Scanning Calorimetry (DSC) is typically used during biopharmaceutical development to characterize a biomolecule through its thermal stability and unfolding profile. Here we demonstrate the suitability of DSC for studies of opaque solutions and slurries. Because of its universal indicator, heat, the technique can be applied to samples with magnetic or polystyrene beads, liposomes, and even chromatographic slurries. Moving out of development, we investigate DSC’s applicability to answer the bioprocessing question: “What is happing inside the column?”. This talk starts to answer this question and highlights previous DSC and ITC work that studied chromatographic efficiency based on changes in thermograms.

Colette Quinn is a Sr Business Development Manager in the Biopharmaceutical group at Waters Corporation. Prior to this she was the microcalorimetry product manager at TA Instruments. She earned her doctorate from Dartmouth College where she spent years keeping cysteines reduced.

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