theCrux
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9:04
Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]
theCrux
66 views • 1 day ago
10:54
Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]
theCrux
160 views • 5 days ago
13:10
Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]
theCrux
277 views • 12 days ago
15:33
Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]
theCrux
234 views • 2 weeks ago
10:00
Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]
theCrux
200 views • 3 weeks ago
13:18
RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)
theCrux
215 views • 1 month ago
7:40
Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning
theCrux
1K views • 3 months ago
14:42
Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning
theCrux
1.3K views • 5 months ago
15:08
TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional
theCrux
3.1K views • 8 months ago
16:58
Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
theCrux
1.1K views • 9 months ago
14:12
Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer
theCrux
3.4K views • 9 months ago
21:45
Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
theCrux
4.3K views • 10 months ago
16:06
P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality
theCrux
1.6K views • 10 months ago
11:51
F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)
theCrux
1.3K views • 10 months ago
15:01
Lambda Phage and Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus
theCrux
5.2K views • 10 months ago
26:22
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
theCrux
3.3K views • 11 months ago
10:35
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
theCrux
1.5K views • 11 months ago
26:59
Restriction Enzymes in Molecular Cloning
theCrux
1.4K views • 11 months ago
6:27
Genetic Engineering - Classic vs Modern methods
theCrux
2.6K views • 11 months ago
9:56
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
theCrux
3.4K views • 1 year ago
8:38
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
theCrux
2K views • 1 year ago
15:18
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)
theCrux
9.8K views • 1 year ago
17:41
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
theCrux
11K views • 1 year ago
15:23
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
theCrux
4.8K views • 1 year ago
12:06
DNA Replication in Space and Time
theCrux
557 views • 1 year ago
18:30
Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation
theCrux
1.5K views • 1 year ago
10:03
DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism
theCrux
2.3K views • 1 year ago
11:42
DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase
theCrux
4.9K views • 1 year ago
12:35
DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome
theCrux
2.3K views • 1 year ago
10:01
DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer
theCrux
2.9K views • 1 year ago
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