Prime editing (pegRNA + RT + nCas9)

Base editing with CRISPR-Cas9

dCas9: CRISPRi CRISPRa CRISPR-ON CRISPR-OFF (Beyond Cas9 Genome Editing)

CRISPR KO vs CRISPR KI experiment using CRISPR-Cas9

How to design and express CRISPR guide RNA [pX330 plasmid, Tutorial]

Genome Editing with CRISPR-Cas9

CRISPR-Cas9-mediated Adaptive Immunity Mechanism in Bacteria [3 Steps of CRISPR-Cas system]

A Brief History of Genome Editing

Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]

Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]

Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]

Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]

Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]

RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)

Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning

TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional

Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning

Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer

Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors

P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality

F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)

Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

Plasmid and Phagemid cloning vector - Selectable vs. Screening marker

Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

Restriction Enzymes (Endonucleases) in Molecular Cloning

Genetic Engineering - Classic vs Modern methods

RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps

Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)

Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)