Published On Nov 26, 2021
In this video I have discussed about Gene Cloning past year questions asked in CSIR and GATE entrance exams.
Please watch Gene Cloning Part 1 here - • GENE CLONING In Detail - Part 1 | CSIR
In molecular biology, cloning a gene means inserting the sequence of the gene into a plasmid, which is a small piece of circular DNA that can be "transfected" into a bacterial strain, where the plasmid will replicate to many, many copies. The plasmid carries an antibiotic resistance gene, so when the bacteria are grown in the presence of the antibiotic, only those bacterial cells with the plasmid in them, can multiply.
This allows molecular biologists to easily propagate and grow vast amounts if the gene, and also to manipulate it in many ways, for example to introduce mutations to study its function, etc.
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