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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy - a 2 minute Preview of the Experimental Protocol

André Gemeinhardt, Matthew P. McDonald, Katharina König, Michael Aigner, Andreas Mackensen, Vahid Sandoghdar
Max Planck Institute for the Science of Light (MPL),; Milligan College, Area of Scientific Learning; Friedrich Alexander University Erlangen-Nuremberg, Department of Physics; Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen, Department of Internal Medicine 5, Hematology and Oncology;

We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.

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