Download the SEM Concepts Handout: https://bit.ly/31bAyv8

This is a discussion of five of the main physical concepts involved in scanning electron microscopy (SEM) – voltage, current, working distance, and the various electron interactions (e.g. the generation of secondary electrons, backscattered electrons, and characteristic x-rays). This video includes a brief introduction on how to simulate beam–specimen interactions using free CASINO Monte Carlo software (https://www.gel.usherbrooke.ca/casino....

A note about the coating layer: a coating layer will help to avoid charging when working with non-conductive or semi-conductive samples. Please carefully consider if going to higher voltages is appropriate for your sample.

There are additional critical concepts to consider when operating an SEM that are not covered in this video, including: dwell time, pixel resolution, how to achieve a good signal-to-noise ratio image/measurement, and how some of these parameters can effect image resolution. These concepts are explained in detail in the book by Goldstein et al 'Scanning Electron Microscopy and X-Ray Microanalysis'.

A complimentary video will be made to discuss additional important concepts and considerations when changing the working distance (i.e. uneven samples, signal collection efficiency), to highlight the limitations when working with different sample types.
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Download the SEM Concepts Handout: https://bit.ly/31bAyv8
Download the Considerations for Optimal SEM Imaging Results Handout: https://bit.ly/2q8gTiQ
Download the SEM Alignment Handout: https://bit.ly/2OgnvDL
Download any of the KNI's Microscopy Handouts: https://bit.ly/2M7I9GK
For more information, view the KNI's Microscopy Lectures: https://bit.ly/2IH6Es8
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Presented by Matthew Sullivan Hunt, PhD
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Disclaimer: This video is intended to serve as supplemental information and cannot replace in-person instrument training. Caltech and KNI are not responsible for how the presented information is translated for use in other facilities.

For information or questions about this video, contact [email protected]